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연세대학교 의과대학 Yonsei Medical Journal Yonsei Medical Journal 제56권 제3호
발행연도
2015.1
수록면
778 - 784 (7page)

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Purpose: To investigate the effect of antifreeze protein (AFP) supplementation on ovarian vitrification and transplantation. Materials and Methods: In this experimentalstudy, we researched a total of 182 ovaries from 4-week-old ICR mice. The equilibration solution included 20% ethylene glycol (EG), and the vitrification solutionincluded 40% EG, 18% Ficoll, and 0.3 M sucrose. Intact ovaries were first suspendedin 1 mL of equilibration solution for 10 min, and then mixed with 0.5 mL of vitrification solution for 5 min. Ovaries were randomly assigned to 3 groups and 0, 5, or 20 mg/mL of type III AFP was added into the vitrification solution (control, AFP5, and AFP20 groups, respectively). The vitrified ovaries were evaluated after warming and 2 weeks after autotransplantation. The main outcome measurements are follicular morphology and apoptosis assessed by histology and the TUNEL assay. Results: A significantly higher intact follicle ratio was shown in the AFP treatedgroups (control, 28.9%; AFP5, 42.3%; and AFP20, 44.7%). The rate of apoptoticfollicles was significantly lower in the AFP treated groups (control, 26.6%; AFP5, 18.7%; and AFP20, 12.6%). After transplantation of the vitrified-warmed ovaries, a significantly higher intact follicle ratio was shown in the AFP20 group. The rate of apoptotic follicles was similar among the groups. Conclusion: The resultsof the present study suggest that supplementing AFP in the vitrification solutionhas beneficial effects on the survival of ovarian tissue during cryopreservation and transplantation.

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