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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 62 - 68 (7page)
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The culture conditions to maximize the production of laccase (EC 1.10.3.2) from Fomitopsis pinicola mycelia were investigated.
Among the tested media for the enzyme production, mushroom complete medium (MCM ; 2% dextrose,0.2% peptone, 0.2% yeast extract, 0.05% KH2PO4, and 0.05% MgSO4 · 7H2O) showed the highest activity of the enzyme. To optimize the culture condition for the laccase activity, influence of various carbon and nitrogen sources was investigated in MCM. Among various carbon and nitrogen sources, 2% glucose and
0.4% peptone showed the highest production of the enzyme, respectively. For the phosphorus and inorganic source, 0.05% NaH2PO4 and 0.05% CaCl2 were best for the enzyme activity. The enzyme production was reached to highest level after the cultivation for 8 days at 25℃. Native polyacrylamide gel electrophoresis (PAGE) followed by the laccase activity staining using 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as the substrate was performed to identify the laccase under culture conditions studied. Zymogram analysis of the culture supernatant showed a laccase band with molecular mass of 52 kDa. The optimum pH and temperature for the enzyme activity were 80℃ and pH 3.0.
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참고문헌 (25)
참고문헌 신청
Ander, P / 1976 / The importance of phenoloxidase activity in lignin degradation by the white-rot fungus Sporotrichum pulberulentum / Arch. Microbiol 109 : 1 ~ 8
Arora, D. S / 2000 / Laccase production by some white rot fungi under different nutritional conditions / Bioresource Technol 73 : 283 ~ 285
Bollg, J. M / 1984 / Comparative studies of extracelluar fungal laccases / Appl. Environ. Microbiol. 48 : 849 ~ 854
Burnpus, J. A / 1989 / Biodegradation of polycyclic aromatic hydrocarbons by Phanerochaete chrysosporium / Appl. Environ. Microbiol 55 (1) : 154 ~ 158
Cambria, M. T / 2000 / Production, Purificatiopn, and Properties of an Extracellular Laccase from Rigidoporus lignosus / Protein Expression and Purification 18 : 141 ~ 147
Arora, D. S / 2000 / Laccase production by some white rot fungi under different nutritional conditions / Bioresource Technol 73 : 283 ~ 285
Bollg, J. M / 1984 / Comparative studies of extracelluar fungal laccases / Appl. Environ. Microbiol. 48 : 849 ~ 854
Burnpus, J. A / 1989 / Biodegradation of polycyclic aromatic hydrocarbons by Phanerochaete chrysosporium / Appl. Environ. Microbiol 55 (1) : 154 ~ 158
Cambria, M. T / 2000 / Production, Purificatiopn, and Properties of an Extracellular Laccase from Rigidoporus lignosus / Protein Expression and Purification 18 : 141 ~ 147
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