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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제11호
- 발행연도
- 2009.1
- 수록면
- 1,295 - 1,300 (6page)
이용수
초록· 키워드
오류제보하기
A 2,172-bp full-length gene (aga-F78), encoding a proteaseresistant
α-galactosidase, was cloned from Rhizopus sp.
F78 and expressed in Escherichia coli. The deduced amino
acid sequence shared highest identity (45.0%) with an α-
galactosidase of glycoside hydrolase family 36 from Absidia
corymbifera. After one-step purification with a Ni-NTA
chelating column, the recombinant Aga-F78 migrated as
a single band of ~82 and ~210 kDa on SDS-PAGE and
nondenaturing gradient PAGE, respectively, indicating
that the native structure of the recombinant Aga-F78 was
a trimer. Exhibiting the similar properties as the authentic
protein, purified recombinant Aga-F78 was optimally
active at 50oC and pH 4.8, highly pH stable over the pH
range 5.0-10.0, more resistant to some cations and proteases,
and had wide substrate specificity (pNPG, melidiose,
raffinose, and stachyose). The recombinant enzyme also
showed good hydrolytic ability to soybean meal, releasing
galactose of 415.58 μg/g soybean meal. When combined
with trypsin, the enzyme retained over 90% degradability
to soybean meal. These favorable properties make Aga-
F78 a potential candidate for applications in the food and
feed industries.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Ademark, P. / 2001 / Multiple α-galactosidases from Aspergillus niger: Purification, characterization and substrate specificities / Enzyme Microb. Technol 29 : 441 ~ 448
Baik, S. H. / 8084 / Molecular cloning and high-level expression in Escherichia coli of fungal α-galactosidase from Abisidia corymbifera IFO8084 / J. Biosci. Bioeng 90 : 168
Bishop, D. F. / 1986 / Human α-galactosidase A: Nucleotide sequence of a cDNA clone encoding the mature enzyme / Proc. Natl. Acad. Sci. U.S.A. 83 : 4859 ~ 4863
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 7
Cao, Y. / 2007 / Purification and characterization of a novel proteaseresistant α-galactosidase from Rhizopus sp. F78 ACCC 30795 / Enzyme Microb. Technol 41 : 835 ~ 841
Baik, S. H. / 8084 / Molecular cloning and high-level expression in Escherichia coli of fungal α-galactosidase from Abisidia corymbifera IFO8084 / J. Biosci. Bioeng 90 : 168
Bishop, D. F. / 1986 / Human α-galactosidase A: Nucleotide sequence of a cDNA clone encoding the mature enzyme / Proc. Natl. Acad. Sci. U.S.A. 83 : 4859 ~ 4863
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 7
Cao, Y. / 2007 / Purification and characterization of a novel proteaseresistant α-galactosidase from Rhizopus sp. F78 ACCC 30795 / Enzyme Microb. Technol 41 : 835 ~ 841
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