지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제14권 제4호
- 발행연도
- 2004.1
- 수록면
- 863 - 867 (5page)
이용수
초록· 키워드
오류제보하기
α-Galactosidase from A. oryzae DR-5 wasinduced in the presence of melibiose, raffinose, galactose, andlocust bean galactomannan. The enzyme was purified tohomogeneity by precipitation with acetone followed byion-exchange chromatography using DEAE-Sephacel. Thepurified enzyme showed a single band in both nondenaturing-PAGE and SDS-PAGE. The enzyme was a glycoprotein innature by activity staining. The molecular weight of thepurified enzyme was 93- 95 kDa by SDS-PAGE. The enzymeexhibited the optimum pH and temperature at 4.7 and60oC, respectively. α-Galactosidase activity was stronglyinhibited by Ag2+, Hg2+, Cu2+, and galactose. EDTA, 1,10-phenanthraline, and PMSF did not inhibit the enzyme activity,whereas N-bromosuccinimide completely inhibited enzymeactivity. Investigation by TLC showed complete hydrolysis ofstachyose and raffinose in soymilk in 3 h at pH 5.0 and 50oC.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
/ 1989197-201 / Enzymic hydrolysis of raffinose and stachyose insoymilk by α-galactosidase from germinating guar
A / 1966 / Effects of various soybean products on flatulence in the adultman : 1235 ~ 1239
A / 1984 / Purificationand enzymatic properties of α-galactosidase from Pycnoporuscinnabarinus : 1319 ~ 1327
Ademark, P / 2001 / Multiple α-galactosidases from Aspergillus niger:Purification, characterization and substrate specificities 29 : 441 ~ 448
Civas, A / 1984 / Glycosidases induced in Aspergillus tamarii : 857 ~ 863
A / 1966 / Effects of various soybean products on flatulence in the adultman : 1235 ~ 1239
A / 1984 / Purificationand enzymatic properties of α-galactosidase from Pycnoporuscinnabarinus : 1319 ~ 1327
Ademark, P / 2001 / Multiple α-galactosidases from Aspergillus niger:Purification, characterization and substrate specificities 29 : 441 ~ 448
Civas, A / 1984 / Glycosidases induced in Aspergillus tamarii : 857 ~ 863
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Production of α- and β-Galactosidases from Bifidobacterium longum subsp. longum RD47
Journal of Microbiology and Biotechnology
2014 .01
-
α-Galactosidase from Bacillus megaterium VHM1 and Its Application in Removal of Flatulence-Causing Factors from Soymilk
Journal of Microbiology and Biotechnology
2010 .01
-
a-Galactosidase를 생산하는 Bacillus licheniformisYB-42의 분리와 효소 특성
한국미생물·생명공학회지
2004 .01
-
청국장 유래 Bacillus licheniformis의 β-Galactosidase 특성
한국미생물·생명공학회지
2012 .01
-
Purification and Characterization of Endo-β-1,4 Mannanase from Aspergillus niger gr for Application in Food Processing Industry
Journal of Microbiology and Biotechnology
2009 .01
-
Enhanced Enzyme Activities of Inclusion Bodies of Recombinantβ-Galactosidase via the Addition of Inducer Analog after L-ArabinoseInduction in the araBAD Promoter System of Escherichia coli
Journal of Microbiology and Biotechnology
2008 .01
-
Degradation of Raffinose Oligosaccharides in Soymilk by Immobilized α-Galactosidase of Aspergillus oryzae
Journal of Microbiology and Biotechnology
2007 .01
-
β-Galactosidase 생산 유산균 선별 및 특성 조사
미생물학회지
2006 .09
-
된장에서 분리된 Bacillus licheniformis의 β-galactosidase 생산성과 효소특성
한국미생물·생명공학회지
2014 .01
-
Aspergillus niger ATCC 16513과 대두(Glycine max. L) $\alpha$-galactosidase의 kinetic 성질
자연 과학 논문집 : 배재대학 첨단과학연구소
1992 .01
-
Enhancement of Sensitivity in Interferometric Biosensing by Using a New Biolinker and Prebinding Antibody
Journal of Microbiology and Biotechnology
2006 .01
-
Bacillus licheniformis로부터 생산된 α-Galactosidase의 가수분해 활성
미생물학회지
2004 .12
-
Molecular Cloning and Expression of a Novel Protease-resistant GH-36 α- Galactosidase from Rhizopus sp. F78 ACCC 30795
Journal of Microbiology and Biotechnology
2009 .01
-
Bacillus licheniformis 분리균 2종의 α-Galactosidase 생산성과효소특성
한국미생물·생명공학회지
2015 .01
-
대두의 발효에 따른 $\alpha$-Galactosidase활성 및 Stachyose, Raffinose 함량 변화
한국식품조리과학회지
1998 .01
-
Weissella cibaria가 생산하는 α-Galactosidase 및 β-Glucosidase의 특성
한국미생물·생명공학회지
2009 .01
-
Molecular Characterization of Cold-Inducible β-Galactosidase from Arthrobacter sp. ON14 Isolated from Antarctica
Journal of Microbiology and Biotechnology
2011 .01
-
Isolation and Characterization of Cold-adapted Strains Producing β-Galactosidase
The Journal of Microbiology
2006 .08
-
Expression and Purification of Recombinant α-Galactosidase in Saccharomyces cerevisiae
한국생명과학회 심포지움
2006 .09
이 논문의 저자 정보
최근 본 자료
댓글(0)
0