지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 32 - 39 (8page)
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초록· 키워드
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Background: Keratinocytes release various pro-inflammatorycytokines, chemokines, and adhesion molecules such asintercellular adhesion molecule 1 (ICAM-1) in response tocytokines such as tumor necrosis factor (TNF)-α and interferon(IFN)-γ. Rapamycin and mycophenolic acid (MPA)have potent immunosuppressive activity because theyinhibit lymphocyte proliferation.
Objective: We investigated the effects of rapamycin andMPA on the expression of inflammation-related factors suchas ICAM-1 and inducible nitric oxide synthase (iNOS),pro-inflammatory cytokines and chemokines, and relatedsignaling pathways in TNF-α-stimulated HaCaT cells.
Methods: The viability of HaCaT cells treated with rapamycinand MPA was confirmed using MTT assay. The expressionof various cytokines such as interleukin (IL)-1β, IL-6,and IL-8; inflammation-related factors such as ICAM-1 andiNOS; and the activation of mitogen activated protein kinase(MAPK) signaling pathways mediated by extracellular signal-related kinases (ERK), p38, and c-Jun N-terminal kinases(JNK) in TNF-α-stimulated HaCaT cells were confirmedusing reverse transcription-polymerase chain reaction andwestern blotting.
Results: Combined treatment of TNF-α-induced HaCaTcells with rapamycin and MPA decreased ICAM-1 and iNOSexpression and ERK and p38 activation more than treatmentwith either drug alone. The most significant decrease wasobserved with a combination of rapamycin (80 nM) and MPA(20 nM). These results show that co-treatment with theseagents has a synergistic anti-inflammatory effect by blockingthe activation of the ERK/p38 MAPK signaling pathway andthus suppressing the TNF-α-induced expression of ICAM-1and iNOS.
Conclusion: The combination of rapamycin and MPA couldpotentially be used as a therapeutic approach in inflammatoryskin diseases.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Albanesi C / 1998 / Interferon-gammastimulated human keratinocytes express the genes necessary for the production of peptide-loaded MHC class II molecules / J Invest Dermatol
Allison AC / 1993 / Immunosuppressive and other effects of mycophenolic acid and an ester prodrug, mycophenolate mofetil / Immunol Rev 136 : 5 ~ 28
Arnott CH / 2004 / Expression of both TNF-alpha receptor subtypes is essential for optimal skin tumour development / Oncogene 23 : 1902 ~ 1910
Bachelez H / 2005 / Immunopathogenesis of psoriasis: recent insights on the role of adaptive and innate immunity / J Autoimmun 25 (Suppl) : 69 ~ 73
Baeuerle PA / 1996 / NF-kappa B: ten years after / Cell 87 : 13 ~ 20
Allison AC / 1993 / Immunosuppressive and other effects of mycophenolic acid and an ester prodrug, mycophenolate mofetil / Immunol Rev 136 : 5 ~ 28
Arnott CH / 2004 / Expression of both TNF-alpha receptor subtypes is essential for optimal skin tumour development / Oncogene 23 : 1902 ~ 1910
Bachelez H / 2005 / Immunopathogenesis of psoriasis: recent insights on the role of adaptive and innate immunity / J Autoimmun 25 (Suppl) : 69 ~ 73
Baeuerle PA / 1996 / NF-kappa B: ten years after / Cell 87 : 13 ~ 20
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