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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 449 - 458 (10page)
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Hepatitis B treatments using immune therapy are gaining interest because of the improvements in dendritic cell performance for antigen presentation, which induces an appropriate immune response and raises patient survival rates. This research aims to produce a significant amount of the HBcAg antigen, which can induce an immune response and have a curative effect on HBV infection. In this study, the HBV subgenotype B3 of the HBcAg gene was used, which is dominant in Indonesia. Further, Lactococcus lactis bacteria was used as the host because of its safety and tightly regulated protein expression. The codon usage for the HBcAg gene was optimized to improve protein expression in L. lactis, which is important because a codon is not random between species. The HBcAg gene is attached to a pNZ8148 plasmid and transformed into the L. lactis NZ3900 expression host. The results confirm that a positive protein band (21 kDa) in two fractions of purified HBcAg was recognized by both western blotting and dot blot hybridization, even if the HBcAg optimized codon has higher GC contents than that suggested for L. lactis expression. Overall, this research strengthens the broad use of L. lactis bacteria for any protein expression, including higher protein expression of codon optimized HBcAg gene compared to non-optimized genes. Furthermore, the improvement in the codon optimization of the HBcAg gene significantly increases the total protein expression by 10–20%, and the expression level of the codon optimized HBcAg increases 1.5 to 3.2-times that of the native HBcAg.
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참고문헌 (29)
참고문헌 신청
World Health Organization / Factsheet No. 204
Thedja MD / 2011 / Ethnogeographical structure of hepatitis B virus genotype distribution in Indonesia and discovery of a new subgenotype, B9 / Arch. Virol 156:855~868
Nurainy N / 2008 / Genetic study of hepatitis B virus in Indonesia reveals a new subgenotype of genotype B in Nusa Tenggara / Arch. Virol 153:1057~1065
Utama A / 2009 / Hepatitis B virus genotypes/subgenotypes in voluntary blood donors in Makassar, South Sulawesi, Indonesia / Virol. J 6:128
Utama A / 2009 / Hepatitis B virus subgenotypes and basal core promoter mutations in Indonesia / World J. Gastroenterol 15:4028~4036
Thedja MD / 2011 / Ethnogeographical structure of hepatitis B virus genotype distribution in Indonesia and discovery of a new subgenotype, B9 / Arch. Virol 156:855~868
Nurainy N / 2008 / Genetic study of hepatitis B virus in Indonesia reveals a new subgenotype of genotype B in Nusa Tenggara / Arch. Virol 153:1057~1065
Utama A / 2009 / Hepatitis B virus genotypes/subgenotypes in voluntary blood donors in Makassar, South Sulawesi, Indonesia / Virol. J 6:128
Utama A / 2009 / Hepatitis B virus subgenotypes and basal core promoter mutations in Indonesia / World J. Gastroenterol 15:4028~4036
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