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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 550 - 555 (6page)
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초록· 키워드
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Purpose: The genus Aeromonas is a pathogen that is well known to cause severe clinical illnesses, ranging from gastroenteritis to sepsis. Accurate identification of A. hydrophila, A. caviae, and A. veronii is important for the care of patients. However, species identification remains difficult using conventional methods. The aim of this study was to compare the accuracy of different methods of identifying Aeromonas at the species level: a biochemical method, matrix-assisted laser desorption ionization mass spectrometry-time of flight (MALDI-TOF MS), 16S rRNA sequencing, and housekeeping gene sequencing (gyrB, rpoB). Materials and Methods: We analyzed65 Aeromonas isolates recovered from patients at a university hospital in Koreabetween 1996 and 2012. The isolates were recovered from frozen states and testedusing the following four methods: a conventional biochemical method, 16S rRNA sequencing, housekeeping gene sequencing with phylogenetic analysis, and MALDI-TOF MS. Results: The conventional biochemical method and 16S rRNA sequencing identified Aeromonas at the genus level very accurately, although species level identification was unsatisfactory. MALDI-TOF MS system correctly identified 60 (92.3%) isolates at the species level and an additional four (6.2%) at the genus level. Overall, housekeeping gene sequencing with phylogenetic analysis was found to be the most accurate in identifying Aeromonas at the species level. Conclusion: The most accurate method of identification of Aeromonas to species level is by housekeeping gene sequencing, although high cost and technical difficulty hinder its usage in clinical settings. An easy-to-use identification method is needed for clinical laboratories, for which MALDI-TOF MS could be a strong candidate.
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등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Abbott SL / 2003 / The genus Aeromonas: biochemical characteristics, atypical reactions, and phenotypic identification schemes / J Clin Microbiol 41 : 2348 ~ 2357
Benagli C / 2012 / A rapid MALDI-TOF MS identification database at genospecies level for clinical and environmental Aeromonas strains / PLoS One 7 : e48441 ~
Blondiaux N / 2010 / MALDI-TOF mass spectrometry to identify clinical bacterial isolates: evaluation in a teaching hospital in Lille / Pathol Biol (Paris) 58 : 55 ~ 57
Chong Y / 1980 / Cultural and biochemical characteristics of clinical isolates of Aeromonas hydrophila / Yonsei Med J 21 : 52 ~ 57
Donohue MJ / 2006 / The development of a matrix-assisted laser desorption/ionization mass spectrometry-based method for the protein fingerprinting and identification of
Benagli C / 2012 / A rapid MALDI-TOF MS identification database at genospecies level for clinical and environmental Aeromonas strains / PLoS One 7 : e48441 ~
Blondiaux N / 2010 / MALDI-TOF mass spectrometry to identify clinical bacterial isolates: evaluation in a teaching hospital in Lille / Pathol Biol (Paris) 58 : 55 ~ 57
Chong Y / 1980 / Cultural and biochemical characteristics of clinical isolates of Aeromonas hydrophila / Yonsei Med J 21 : 52 ~ 57
Donohue MJ / 2006 / The development of a matrix-assisted laser desorption/ionization mass spectrometry-based method for the protein fingerprinting and identification of
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