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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 1,226 - 1,236 (11page)
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Ovotransferrin (OTF) is a well-known protein of the transferrin family with strong iron chelating activity, resulting in its antimicrobial activity. Furthermore, OTF is known to have antioxidant, anticancer, and antihypertensive activities. However, there have been few studies about the immune-enhancing activity of OTF. In current study, we investigated the immune-enhancing activity of OTF using the murine macrophage cells in vitro. The effect of OTF on production of pro-inflammatory mediators and cytokines were determined using Griess assay and quantitative real-time PCR. Using Neutral Red uptake assay, we confirmed the effect of OTF on phagocytic activity of macrophages. Ovotransferrin significantly increased the production of nitric oxide (NO) and secretion of inducible nitric oxide synthase (iNOS) mRNA with no cytotoxic activity. Ovotransferrin (2 mg/mL) stimulated NO production up to $31.9{\pm}3.5{\mu}M$. Ovotransferrin significantly increased the mRNA expression levels of pro-inflammatory cytokines which are tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), Interleukin-$1{\beta}$ (IL-$1{\beta}$), and IL-6: OTF (2 mg/mL) treatment increased the secretion of mRNA for TNF-${\alpha}$, IL-$1{\beta}$, and IL-6 by 22.20-, 37.91-, and 6.17-fold of the negative control, respectively. The phagocytic activity of macrophages was also increased by OTF treatment significantly compared with negative control. Also, OTF treatment increased phosphorylation level of MAPK signaling pathways. These results indicated that OTF has immune-enhancing activity by activating RAW 264.7 macrophages via MAPK pathways.
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참고문헌 (39)
참고문헌 신청
Abdallah FB / 1999 / Transferrins, the mechanism of iron release by ovotransferrin / Eur J Biochem 263:912~920
Abeyrathne ED / 2013 / Separation of ovotransferrin from chicken egg white without using organic solvents / Poult Sci 92:1091~1097
Ahmad W / 2018 / Immunomodulatory effects of Tinospora crispa extract and its major compounds on the immune functions of RAW 264.7 macrophages / Int Immunopharmacol 60:141~151
Cameron DJ / 1980 / Cytotoxicity of human macrophages for tumor cells: Enhancement by bacterial lipopolysaccharides (LPS) / J Immunol 124:708~712
Castro R / 2008 / Resveratrol modulates innate and inflammatory responses in fish leucocytes / Vet Immunol Immunopathol 126:9~19
Abeyrathne ED / 2013 / Separation of ovotransferrin from chicken egg white without using organic solvents / Poult Sci 92:1091~1097
Ahmad W / 2018 / Immunomodulatory effects of Tinospora crispa extract and its major compounds on the immune functions of RAW 264.7 macrophages / Int Immunopharmacol 60:141~151
Cameron DJ / 1980 / Cytotoxicity of human macrophages for tumor cells: Enhancement by bacterial lipopolysaccharides (LPS) / J Immunol 124:708~712
Castro R / 2008 / Resveratrol modulates innate and inflammatory responses in fish leucocytes / Vet Immunol Immunopathol 126:9~19
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