지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
The genus Pediococcus belongs to the lactic acid bacteria and includes 15 species which are used in the food industry as both starter and probiotic cultures. The importance of Pediococcus spp. is due to their use as starter cultures in fermented meat as well as to their presence as the natural microbiota in vegetables. The availability of P. pentosaceus in the food industry increases the need for reliable molecular techniques for strain identification. To date, the reliable molecular methods for definite identification at strain level of microorganisms used in food industry has not been developed. Molecular identification based on suitable marker genes could be a promising alternative to conventional molecular typing methods such as ribotyping. In this study, the applicability of seven housekeeping genes gyrB, pyc, pgm, leuS, glnA, and dalR in combination with the pgi gene in multilocus sequence typing of P. pentosaceus was assessed. Sequencing and comparative analysis of sequence data were performed on 6 strains isolated from various vegetables. In addition to 17 sequence types, two new sequence types were identified and these fortified sequence types and seven marker genes allowed for a clear differentiation of the strains analyzed, indicating their applicability in molecular typing.
목차
등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Dunbar J. / 2001 / Phylogenetic specificity and reproducibility and new method for analysis of terminal restriction fragment profiles of 16S rRNA genes from bacterial communities / Appl. Environ. Microbiol 67:190~197
Gancheva A. / 1999 / A polyphasic approach towards the identification of strains belonging to Lactobacillus acidophilus and related species / Syst. Appl. Microbiol 22:573~585
Gevers D. / 2001 / Applicability of rep-PCR fingerprinting for identification of lactobacillus species / FEMS Microbiol. Lett 205:31~36
Healy M. / 2005 / Microbial DNA typing by automated repetitive-sequence-based PCR / J. Clin. Microbiol 43:199~207
Higgins CF. / 1982 / A novel intercistronic regulatory element of prokaryotic operons / Nature 298:760~762
Gancheva A. / 1999 / A polyphasic approach towards the identification of strains belonging to Lactobacillus acidophilus and related species / Syst. Appl. Microbiol 22:573~585
Gevers D. / 2001 / Applicability of rep-PCR fingerprinting for identification of lactobacillus species / FEMS Microbiol. Lett 205:31~36
Healy M. / 2005 / Microbial DNA typing by automated repetitive-sequence-based PCR / J. Clin. Microbiol 43:199~207
Higgins CF. / 1982 / A novel intercistronic regulatory element of prokaryotic operons / Nature 298:760~762
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