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The effect of temperature preconditioning on skin blackening disorder observed in 'Niitaka' pear (Pyrus pyrifolia) fruits during low temperature storage was investigated. In addition to incidence and severity of blackening, mineral content, changes in fruit fresh weight, firmness, total soluble solids, and titratable acidity were evaluated. The blackening was observed on the epidermis, but not in lenticels, cuticle, or flesh. Skin blackening appeared in more than 62% of pear fruits stored for more than 15 days below 1℃. Preconditioning at 10℃ for 10 days or gradual cooling (reducing 1℃ per day from 15 to 1℃) prior to storage at 1℃ reduced blackening disorder to 3.7 and 0.2%, respectively. Moreover, the blackening disorder did not occur at 10℃ storage. A high relative humidity (ranging from 92 to 95%) treatment at 1°C resulted in greater severity of skin blackening than a lower relative humidity treatment (ranging 62 to 82%) after 15 days storage. However, the effect of humidity on blackening was negligible after 30 days. Ca contents in non-blackened fruit flesh and peel were higher than those in fruit that showed blackening. The flesh weight and firmness of blackened fruit were lower compared to normal pear fruit controls. There were no significant differences in dry weight, soluble solids, or total acidity among the treatments. The results suggest that skin blackening of 'Niitaka' pear fruits during low temperature storage is a chilling injury symptom.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Conclusions
Literature Cited

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UCI(KEPA) : I410-ECN-0101-2009-525-016949001