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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.6
- 수록면
- 732 - 738 (7page)
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An acidic polygalacturonase (PG) from Aspergillus kawachii was produced by solid state fermentation employing a polyurethane foam support. The conditions used for the production of acidic PG were particle size of support (0.6 or 500 ㎣) and fermentation time. From the factors studied, the particle size had important influence on enzyme production. The best conditions for acidic PG production were 0.6 ㎣ particle size, 18 hr at 30℃ and initial pH of 5.0. In addition, pectin was extracted from citrus pomaces (grapefruit, lime, and tangerine) by acidic PG at 50℃ for 24 hr with citric acid solution. Infrared spectroscopy showed that lime pomace had more high-methoxylated (65%) endogenous pectin than was obtained than from grapefruit or tangerine pomaces. The enzymatically extracted pectin yield in dry basis (d.b.) for grapefruit and lime pectins were 6.95 and 4.25%, respectively. The citric acid solution alone also contributed to pectin extraction from citrus pomaces (7-9%, d.b.). Limited pectin extraction by acidic PG from tangerine pomace was most likely due to the presence of low-methoxylated endogenous pectin. The enzymatic method for pectin extraction using acidic PG from A. kawachii is a promising technique for releasing highly polymerized pectic substances from high-methoxylated lime or grapefruit pomaces.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgments
References
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