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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 125 - 132 (8page)
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In
order to develop probiotics which may be a viable alternative of antibiotic use in pig industry, five bacterial
strains (Lactobacillus sp. HN 52, 92, 98, 235 and AP 116) possessing antimicrobial properties were selected
from 500 strains isolates of pig intestines. The bacteriocin produced by Lactobacillus sp. HN 235 displayed a
relative broad spectrum of inhibitory activity against all Enterococcus strains, Pseudomonas aeruginosa, Listeria
monocytogenes and Clostridium perfringens using the spot-on-lawn method. The production of antimicrobial
substance started in the middle of the exponential growth phase, reached maximum levels (6,400 AU/
mL) in the stationary phase, and then declined. Bacteriocin activity remained unchanged after 30 min of heat
treatment at 95℃ and stable from pH 2.0 to 10 for 1 h, or exposure to organic solvents; however, it diminished
after treatment with proteolytic enzymes. The molecular weight of the bacteriocin was about 5 kDa according
to a tricine SDS-PAGE analysis.
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등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Aarnisalo, K / 2008 / Use of results of microbiological analyses for riskbased control of Listeria monocytogenes in marinated broiler legs. Int / J. Food Microbiol 121 : 275
Aasen, I. M / 2678 / Influence of complex nutrients, temperature and pH on bacteriocin production by Lactobacillus sakei CCUG 42678 / Appl. Microbiol. Biotechnol 53 : 159 ~ 1
Barefoot, S. F / 1984 / Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B. Antimicrob / Agents Chemother 26 : 328 ~ 334
Byun, J. W. / 2000 / In vitro selection of lactic acid bacteria for probiotic use in pigs / Korea J. Vet. Res. 40 : 701 ~ 706
Carolissen-Mackay, V / 1997 / Purification of bacteriocins of lactic acid bacteria: problems and pointers / Int. J. Food Microbiol 34 : 1 ~ 16
Aasen, I. M / 2678 / Influence of complex nutrients, temperature and pH on bacteriocin production by Lactobacillus sakei CCUG 42678 / Appl. Microbiol. Biotechnol 53 : 159 ~ 1
Barefoot, S. F / 1984 / Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B. Antimicrob / Agents Chemother 26 : 328 ~ 334
Byun, J. W. / 2000 / In vitro selection of lactic acid bacteria for probiotic use in pigs / Korea J. Vet. Res. 40 : 701 ~ 706
Carolissen-Mackay, V / 1997 / Purification of bacteriocins of lactic acid bacteria: problems and pointers / Int. J. Food Microbiol 34 : 1 ~ 16
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