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자료유형
학술저널
저자정보
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제8호
발행연도
2007.1
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1,369 - 1,378 (10page)

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For effective exopolysaccharide production andmycelial growth by a liquid culture of Fomitopsis pinicola inan air-lift bioreactor, the culture temperature, pH, carbon source,nitrogen source, and mineral source were initially investigatedin a flask. The optimal temperature and pH for mycelialgrowth and exopolysacharide production were 25oC and 6.0,respectively. Among the various carbon sources tested,glucose was found to be the most suitable carbon source. Inproduction were achieved in 4% glucose. The best nitrogensources were yeast extract and malt extract. The optimalconcentrations of yeast extract and malt extract were 0.5 and0.1%, respectively. K2HPO4 and MgSO4·7H2O were found tobe the best mineral sources for mycelial growth andexopolysaccharide production. In order to investigate the effect ofaeration on mycelial growth and exopolysaccharide production inmaximum mycelial growth and exopolysaccharide productionwere 7.9 g/l and 2.6 g/l, respectively, at 1.5 vvm of aeration.In addition, a batch culture in an air-lift bioreactor was carried outfor 11 days under the optimal conditions. The maximum mycelialgrowth was 10.4 g/l, which was approximately 1.7-fold higherthan that of basal medium. The exopolysacharide productionwas increased with increased culture time. The maximumconcentration of exopolysaccharide was 4.4 g/l, which was aboutthat exopolysaccharide production increased in paralel withthe growth of mycelium, and also show that product formationis associated with mycelial growth. The developed model in anair-lift bioreactor showed good agreement with experimental dataand simulated results on mycelial growth and exopolysaccharideproduction in the culture of F. pinicola.

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