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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제10호
- 발행연도
- 2017.1
- 수록면
- 1,855 - 1,866 (12page)
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초록· 키워드
오류제보하기
The synergistic activation mediator (SAM) system can robustly activate endogenous gene expression by a single-guide RNA. This transcriptional modulation has been shown to enhance gene promoter activity and leads to epigenetic changes. Human interferon-γ is a common natural glycoprotein involved in antiviral effects and inhibition of cancer cell growth.
Large quantities of high-purity interferon-γ are important for medical research and clinical therapy. To investigate the possibility of employing the SAM system to enhance endogenous human interferon-γ with normal function in innate immunity, we designed 10 single-guide RNAs that target 200 bp upstream of the transcription start sites of the interferon-γ genome, which could significantly activate the interferon-γ promoter reporter. We confirmed that the system can effectively and highly activate interferon-γ expression in several humanized cell lines. Moreover, we found that the interferon-γ induced by the SAM system could inhibit tumorigenesis. Taken together, our results reveal that the SAM system can modulate epigenetic traits of non-immune cells through activating interferon-γ expression and triggering JAK-STAT signaling pathways. Thus, this strategy could offer a novel approach to inhibit tumorigenesis without using exogenous interferon-γ.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Arakawa T / 1987 / Acid unfolding and self-association of recombinant Escherichia coli derived human interferon-gamma / Biochemistry 26 : 5428 ~ 5432
Chawla-Sarkar M / 2003 / Apoptosis and interferons: role of interferon-stimulated genes as mediators of apoptosis / Apoptosis 8 : 237 ~ 249
Chen YJ / 2005 / Development of a bicistronic baculovirus expression vector by the Rhopalosiphum padi virus 5’ internal ribosome entry site / Biochem. Biophys. Res. Commun 3
Chin YE / 1996 / Cell growth arrest and induction of cyclin-dependent kinase inhibitor p21WAF1/CIP1 mediated by STAT1 / Science 272 : 719 ~ 722
Doudna JA / 2014 / Genome editing. The new frontier of genome engineering with CRISPR-Cas9 / Science 346 : 1258096 ~
Chawla-Sarkar M / 2003 / Apoptosis and interferons: role of interferon-stimulated genes as mediators of apoptosis / Apoptosis 8 : 237 ~ 249
Chen YJ / 2005 / Development of a bicistronic baculovirus expression vector by the Rhopalosiphum padi virus 5’ internal ribosome entry site / Biochem. Biophys. Res. Commun 3
Chin YE / 1996 / Cell growth arrest and induction of cyclin-dependent kinase inhibitor p21WAF1/CIP1 mediated by STAT1 / Science 272 : 719 ~ 722
Doudna JA / 2014 / Genome editing. The new frontier of genome engineering with CRISPR-Cas9 / Science 346 : 1258096 ~
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