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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Background: Delayed bone regeneration of fractures in osteoporosis patients or of critical-size bone defects after tumor resection are a major medical and socio-economic challenge. Therefore, the development of more effective and osteoinductive biomaterials is crucial. Methods: We examined the osteogenic potential of macroporous scaffolds with varying pore sizes after biofunctionalization with a collagen/high-sulfated hyaluronan (sHA3) coating in vitro. The three-dimensional scaffolds were made up from a biodegradable three-armed lactic acid-based macromer (TriLA) by crosspolymerization. Templating with solid lipid particles that melt during fabrication generates a continuous pore network. Human mesenchymal stem cells (hMSC) cultivated on the functionalized scaffolds in vitro were investigated for cell viability, production of alkaline phosphatase (ALP) and bone matrix formation. Statistical analysis was performed using student’s t-test or two-way ANOVA. Results: We succeeded in generating scaffolds that feature a significantly higher average pore size and a broader distribution of individual pore sizes (HiPo) by modifying composition and relative amount of lipid particles, macromer concentration and temperature for cross-polymerization during scaffold fabrication. Overall porosity was retained, while the scaffolds showed a 25% decrease in compressive modulus compared to the initial TriLA scaffolds with a lower pore size (LoPo). These HiPo scaffolds were more readily coated as shown by higher amounts of immobilized collagen (+ 44%) and sHA3 (+ 25%) compared to LoPo scaffolds. In vitro, culture of hMSCs on collagen and/or sHA3-coated HiPo scaffolds demonstrated unaltered cell viability. Furthermore, the production of ALP, an early marker of osteogenesis (+ 3-fold), and formation of new bone matrix (+ 2.5-fold) was enhanced by the functionalization with sHA3 of both scaffold types. Nevertheless, effects were more pronounced on HiPo scaffolds about 112%. Conclusion: In summary, we showed that the improvement of scaffold pore sizes enhanced the coating efficiency with collagen and sHA3, which had a significant positive effect on bone formation markers, underlining the promise of using this material approach for in vivo studies.
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참고문헌 (54)
참고문헌 신청
Unnanuntana A / 2010 / The assessment of fracture risk / J Bone Joint Surg Am 92(3):743~753
Camargo WA / 2017 / Diabetes mellitus and bone regeneration : a systematic review and meta-analysis of animal studies / Tissue Eng Part B Rev 23(5):471~479
Rachner TD / 2011 / Osteoporosis : now and the future / Lancet 377(9773):1276~1287
Khosla S / 2017 / Osteoporosis treatment : recent developments and ongoing challenges / Lancet Diabetes Endocrinol 5(11):898~907
Stevens MM / 2008 / Biomaterials for bone tissue engineering / Mater Today 11(5):18~25
Camargo WA / 2017 / Diabetes mellitus and bone regeneration : a systematic review and meta-analysis of animal studies / Tissue Eng Part B Rev 23(5):471~479
Rachner TD / 2011 / Osteoporosis : now and the future / Lancet 377(9773):1276~1287
Khosla S / 2017 / Osteoporosis treatment : recent developments and ongoing challenges / Lancet Diabetes Endocrinol 5(11):898~907
Stevens MM / 2008 / Biomaterials for bone tissue engineering / Mater Today 11(5):18~25
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