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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 48 - 53 (6page)
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Reactive oxygen species (ROS) are widely generated in biological processes such as normal metabolism and response to xenobiotic exposure. While ROS can be beneficial or harmful to cells and tissues, generation of ROS by diverse anti-cancer drugs or phytochemicals plays an important role in the induction of apoptosis. We recently identified a derivative of naphthalene, MS-5, that induces apoptosis of an ovarian cell, CAOV-3. Interestingly, MS-5 induced apoptosis by down-regulating the ROS. Cell viability was evaluated by water-soluble tetrazolium salt (WST-1) assay. Apoptosis was evaluated by flow cytometry analysis. Intracellular ROS ($H_2O_2$), mitochondrial superoxide, mitochondrial membrane potential (MMP) and effect on cycle were determined by flow cytometry. Protein expression was assessed by western blotting. The level of ATP was measured using ATP Colorimetric/Fluorometric Assay kit. MS-5 inhibited growth of ovarian cancer cell lines, CAOV-3, in a concentration- and time-dependent manner. MS-5 also induced G1 cell cycle arrest in CAOV-3 cells, while MS-5 decreased intracellular ROS generation. In addition, cells treated with MS-5 showed the decrease in MMP and ATP production. In this study, we found that treatment with MS-5 in CAOV-3 cells induced apoptosis but decreased ROS level. We suspect that MS-5 might interfere with the minimum requirements of ROS for survival. These perturbations appear to be concentration-dependent, suggesting that MS-5 may induce apoptosis by interfering with ROS generation. We propose that MS-5 may be a potent therapeutic agent for inducing apoptosis in ovarian cancer cell through regulation of ROS.
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참고문헌 (25)
참고문헌 신청
Biliński T / 1989 / Superoxide dismutase deficiency and the toxicity of the products of auto-oxidation of polyunsaturated fatty acids in yeast / Biochim. Biophys. Acta 1001:102~106
Buolamwini JK / 2000 / Cell cycle molecular targets in novel anticancer drug discovery / Curr. Pharm. Des 6:379~392
Cabiscol E / 2000 / Oxidative stress promotes specific protein damage in Saccharomyces cerevisiae / J. Biol. Chem 275:27393~27398
Choi JH / 2007 / Gonadotropins and ovarian cancer / Endocr. Rev 28:440~461
Dickson MA / 2009 / Development of cell-cycle inhibitors for cancer therapy / Curr. Oncol 16:36~43
Buolamwini JK / 2000 / Cell cycle molecular targets in novel anticancer drug discovery / Curr. Pharm. Des 6:379~392
Cabiscol E / 2000 / Oxidative stress promotes specific protein damage in Saccharomyces cerevisiae / J. Biol. Chem 275:27393~27398
Choi JH / 2007 / Gonadotropins and ovarian cancer / Endocr. Rev 28:440~461
Dickson MA / 2009 / Development of cell-cycle inhibitors for cancer therapy / Curr. Oncol 16:36~43
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