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논문 기본 정보

자료유형
학술저널
저자정보
김현복 (농촌진흥청) 고은지 (강원대학교) 류병렬 (강원대학교) 양수진 (강원대학교) 백종섭 (강원대학교) 유수지 (강원대학교) 이현태 (동의대학교) 권진우 (가톨릭대학교) 임정대 (강원대학교)
저널정보
한국약용작물학회 한국약용작물학회지 한국약용작물학회지 제29권 제3호
발행연도
2021.6
수록면
173 - 186 (14page)
DOI
10.7783/KJMCS.2021.29.3.173

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Background: The present study investigated the inhibitory effect of purified mulberry anthocyanin using PB-600 macroporous resin (RPA) in a 3T3-L1 preadipocyte differentiation model and a process for the industrial preparation of mulberry anthocyanins as a natural food and medicine materials.
Methods and Results: Compared with the experimental purification using C18 Sep-Pak cartridges, RPA could yield a significantly higher amount of anthocyanins by an increased loading amount, the amount of purified anthocyanin, and cyanidin-3-glucoside (C3G) purified specifically, decreasing impurities containing cyanidin-3-rutinoside (C3R) in mulberry extract. To investigate the effect of RPA on cytotoxicity and adipogenesis in vitro, differentiating 3T3-L1 cells in adipocyte-induction media were treated with RPA at various concentrations (50 to 2,000 ㎍/㎖). We found that RPA inhibited 3T3-L1 adipocyte differentiation without affecting cell viability, while RPA (1,000 ㎍/㎖) treatment reduced the lipid accumulation inhibition rate to 46.44 ± 2.63%. Additionally, the triglyceride content of RPA (1,000 ㎍/㎖)-treated 3T3-L1 adipocytes decreased to 59.32 ± 1.36% compared to the control (100%). Measuring the anti-obesity effect and gene expression related to lipid metabolism through real-time PCR and experiments were performed at a concentration of less than 1,000 ㎍/㎖. RPA containing anthocyanin and non-anthocyanin fravonoid affects the gene expression of mRNA (SREBP-1c, PPAR, C/EBPα, FAS, and ACC). RPA containing both anthocyanin (C3G and C3R) and non-anthocyanin flavonoid decreased to a higher level than C3G alone in the gene expression of lipid metabolism-related genes, indicating that the lipid metabolism-related genes were significantly reduced.
Conclusions: The macroporus PB-600 resin was used to separate anthocyanin in the extract of mulberry, confirming it as an efficient potential method for the industrial production of mulberry anthocyanins. These results suggest that RPA could have a beneficial effect on the inhibition of adipogenesis, and that it may be used as effective anti-obesity agents.

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UCI(KEPA) : I410-ECN-0101-2021-524-001848614