지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.6
- 수록면
- 173 - 186 (14page)
- DOI
- 10.7783/KJMCS.2021.29.3.173
이용수
초록· 키워드
오류제보하기
Background: The present study investigated the inhibitory effect of purified mulberry anthocyanin using PB-600 macroporous resin (RPA) in a 3T3-L1 preadipocyte differentiation model and a process for the industrial preparation of mulberry anthocyanins as a natural food and medicine materials.
Methods and Results: Compared with the experimental purification using C18 Sep-Pak cartridges, RPA could yield a significantly higher amount of anthocyanins by an increased loading amount, the amount of purified anthocyanin, and cyanidin-3-glucoside (C3G) purified specifically, decreasing impurities containing cyanidin-3-rutinoside (C3R) in mulberry extract. To investigate the effect of RPA on cytotoxicity and adipogenesis in vitro, differentiating 3T3-L1 cells in adipocyte-induction media were treated with RPA at various concentrations (50 to 2,000 ㎍/㎖). We found that RPA inhibited 3T3-L1 adipocyte differentiation without affecting cell viability, while RPA (1,000 ㎍/㎖) treatment reduced the lipid accumulation inhibition rate to 46.44 ± 2.63%. Additionally, the triglyceride content of RPA (1,000 ㎍/㎖)-treated 3T3-L1 adipocytes decreased to 59.32 ± 1.36% compared to the control (100%). Measuring the anti-obesity effect and gene expression related to lipid metabolism through real-time PCR and experiments were performed at a concentration of less than 1,000 ㎍/㎖. RPA containing anthocyanin and non-anthocyanin fravonoid affects the gene expression of mRNA (SREBP-1c, PPAR, C/EBPα, FAS, and ACC). RPA containing both anthocyanin (C3G and C3R) and non-anthocyanin flavonoid decreased to a higher level than C3G alone in the gene expression of lipid metabolism-related genes, indicating that the lipid metabolism-related genes were significantly reduced.
Conclusions: The macroporus PB-600 resin was used to separate anthocyanin in the extract of mulberry, confirming it as an efficient potential method for the industrial production of mulberry anthocyanins. These results suggest that RPA could have a beneficial effect on the inhibition of adipogenesis, and that it may be used as effective anti-obesity agents.
Methods and Results: Compared with the experimental purification using C18 Sep-Pak cartridges, RPA could yield a significantly higher amount of anthocyanins by an increased loading amount, the amount of purified anthocyanin, and cyanidin-3-glucoside (C3G) purified specifically, decreasing impurities containing cyanidin-3-rutinoside (C3R) in mulberry extract. To investigate the effect of RPA on cytotoxicity and adipogenesis in vitro, differentiating 3T3-L1 cells in adipocyte-induction media were treated with RPA at various concentrations (50 to 2,000 ㎍/㎖). We found that RPA inhibited 3T3-L1 adipocyte differentiation without affecting cell viability, while RPA (1,000 ㎍/㎖) treatment reduced the lipid accumulation inhibition rate to 46.44 ± 2.63%. Additionally, the triglyceride content of RPA (1,000 ㎍/㎖)-treated 3T3-L1 adipocytes decreased to 59.32 ± 1.36% compared to the control (100%). Measuring the anti-obesity effect and gene expression related to lipid metabolism through real-time PCR and experiments were performed at a concentration of less than 1,000 ㎍/㎖. RPA containing anthocyanin and non-anthocyanin fravonoid affects the gene expression of mRNA (SREBP-1c, PPAR, C/EBPα, FAS, and ACC). RPA containing both anthocyanin (C3G and C3R) and non-anthocyanin flavonoid decreased to a higher level than C3G alone in the gene expression of lipid metabolism-related genes, indicating that the lipid metabolism-related genes were significantly reduced.
Conclusions: The macroporus PB-600 resin was used to separate anthocyanin in the extract of mulberry, confirming it as an efficient potential method for the industrial production of mulberry anthocyanins. These results suggest that RPA could have a beneficial effect on the inhibition of adipogenesis, and that it may be used as effective anti-obesity agents.
목차
ABSTRACT
서언
재료 및 방법
결과 및 고찰
REFFERENCES
참고문헌 (53)
참고문헌 신청
Ailhaud G / 1992 / Cellular and molecular aspects of adipose tissue development / Annual Review of Nutrition 12:207~233
Ali AT / 2013 / Adipocyte and adipogenesis / European Journal of Cell Biology 92:229~236
Babizhayev MA / 1994 / L-carnosine(β-alanyl-L-histidine)and carcinine(β-alanylhistamine)act as natural antioxidants with hydroxyl radical-scavening and lipidperoxidase activities / Biochemical Journal 304:509~516
방인수 / 2010 / Effects of Temperature and UV Irradiation on Stability of Anthocyanin-Polyphenol Copigment Complex in Mulberry Fruits / 한국약용작물학회지 18(3):191~200
Bartelt A / 2014 / Adipose tissue browning and metabolic health / Nature Reviews Endocrinology 10:24~36
Ali AT / 2013 / Adipocyte and adipogenesis / European Journal of Cell Biology 92:229~236
Babizhayev MA / 1994 / L-carnosine(β-alanyl-L-histidine)and carcinine(β-alanylhistamine)act as natural antioxidants with hydroxyl radical-scavening and lipidperoxidase activities / Biochemical Journal 304:509~516
방인수 / 2010 / Effects of Temperature and UV Irradiation on Stability of Anthocyanin-Polyphenol Copigment Complex in Mulberry Fruits / 한국약용작물학회지 18(3):191~200
Bartelt A / 2014 / Adipose tissue browning and metabolic health / Nature Reviews Endocrinology 10:24~36
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