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논문 기본 정보

자료유형
학술저널
저자정보
Cheryl C. Adeva (Department of Agronomy Chungnam National University) 이현숙 (충남대학교) 김선하 (충남대학교) 전윤아 (Department of Agronomy Chungnam National University) 심규찬 (Department of Agronomy Chungnam National University) Ngoc Ha Luong (Chungnam National University) 강주원 (Department of Southern Area Crop Science National Institute of Crop Science RDA) 김창수 (Department of Agronomy Chungnam National University) 조준현 (Department of Southern Area Crop Science National Institute of Crop Science RDA) 안상낙 (Department of Agronomy Chungnam National University)
저널정보
한국육종학회 Plant Breeding and Biotechnology Plant Breeding and Biotechnology Vol.8 No.4
발행연도
2020.1
수록면
354 - 367 (14page)

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Quantitative trait loci (QTLs) for the starch-related traits amylose content (AC) and resistant starch (RS) content havereceived much attention due to the potential benefits of grains high in these starch levels. In this study, QTLs associated with AC andRS content were identified using 92 recombinant inbred lines (RILs) developed from a cross between two closely related japonicacultivars ‘Dodamssal’ and ‘Hwayeong’. One QTL on chromosome 2 for RS content and 2 QTLs for AC on chromosomes 2 and 6 weredetected. The F2 population derived from a cross between Hwayeong and two selected RILs were used to analyze the interactionbetween starch branching enzyme 3 (SBE3) and granule-bound starch synthase 1 (GBSS1). The combined effect of SBE3 and GBSS1 inthe F2 population suggested that these two genes behaved in an additive manner in increasing AC. Haplotype analysis based on twoSNPs in GBSS1 classified 117 rice accessions into three groups. At the first SNP site, all indica, Korean landrace, and weedy riceaccessions had the Wxaallele at the 5ʹ splice site of intron 1, whereas japonica accessions had the mutated Wxballele. This suggests thatthis splice-donor mutation is prevalent in japonica cultivars, but rare or absent in landrace and weedy rice cultivars. Landrace or weedyrice accessions harboring the Wxaallele could be employed in breeding programs to manipulate AC in cultivated japonica riceconsidering the difficulty and time to introduce desirable indica traits into japonica due to reproductive barriers.

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