지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.10
- 수록면
- 34 - 49 (16page)
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초록· 키워드
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Sjögren"s syndrome (SS) is an autoimmune disease characterized by dryness of the mouth and eyes. The glandular dysfunction in SS involves not only T cell-mediated destruction of the glands but also autoantibodies against the type 3 muscarinic acetylcholine receptor or aquaporin 5 (AQP5) that interfere with the secretion process. Studies on the breakage of tolerance and induction of autoantibodies to these autoantigens could benefit SS patients. To break tolerance, we utilized a PmE-L peptide derived from the AQP5-homologous aquaporin of Prevotella melaninogenica (PmAqp) that contained both a B cell “E” epitope and a T cell epitope. Repeated subcutaneous immunization of C57BL/6 mice with the PmE-L peptide efficiently induced the production of Abs against the “E” epitope of mouse/human AQP5 (AQP5E), and we aimed to characterize the antigen specificity, the sequences of AQP5Especific B cell receptors, and salivary gland phenotypes of these mice. Sera containing anti-AQP5E IgG not only stained mouse Aqp5 expressed in the submandibular glands but also detected PmApq and PmE-L by immunoblotting, suggesting molecular mimicry. Characterization of the AQP5E-specific autoantibodies selected from the screening of phage display Ab libraries and mapping of the B cell receptor repertoires revealed that the AQP5E-specific B cells acquired the ability to bind to the Ag through cumulative somatic hypermutation. Importantly, animals with anti-AQP5E Abs had decreased salivary flow rates without immune cell infiltration into the salivary glands. This model will be useful for investigating the role of anti-AQP5 autoantibodies in glandular dysfunction in SS and testing new therapeutics targeting autoantibody production.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (36)
참고문헌 신청
Thorne I / 2017 / Sjögren's syndrome / Br J Hosp Med(Lond) 78:438~442
Christodoulou MI / 2010 / Characteristics of the minor salivary gland infiltrates in Sjögren's syndrome / J Autoimmun 34:400~407
Routsias JG / 2007 / Sjögren's syndrome--study of autoantigens and autoantibodies / Clin Rev Allergy Immunol 32:238~251
Scofield RH / 2018 / Prognostic value of Sjögren's syndrome autoantibodies / J Lab Precis Med 3:92
Alam J / 2016 / Detection of autoantibodies against aquaporin-5 in the sera of patients with primary Sjögren's syndrome / Immunol Res 64:848~856
Christodoulou MI / 2010 / Characteristics of the minor salivary gland infiltrates in Sjögren's syndrome / J Autoimmun 34:400~407
Routsias JG / 2007 / Sjögren's syndrome--study of autoantigens and autoantibodies / Clin Rev Allergy Immunol 32:238~251
Scofield RH / 2018 / Prognostic value of Sjögren's syndrome autoantibodies / J Lab Precis Med 3:92
Alam J / 2016 / Detection of autoantibodies against aquaporin-5 in the sera of patients with primary Sjögren's syndrome / Immunol Res 64:848~856
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UCI(KEPA) : I410-ECN-0101-2022-517-000121217