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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.12
- 수록면
- 433 - 444 (12page)
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This study was conducted to biochemically characterize and screen the efficacy of antagonistic bacterial isolates in suppressing sclerotinia rot in lettuce crops. A total of 123 bacterial isolates were isolated from soil. In vitro antagonistic screening showed that 13 bacterial isolates were effective against Sclerotinia sclerotiorum and S. minor. In particular, two strains of Bacillus megaterium (HCS-1B and WJR-6B) showed the highest effectiveness against the tested pathogens, with a 75–80% inhibition rate. Additionally, the 13 bacterial isolates were biochemically characterized. Ammonia production, phosphate solubilization, protease production, starch hydrolysis and siderophore production, and HCN production were observed in 13, 3, 11, 9, and 2 bacterial isolates, respectively. Based on the results of the in vitro antagonistic test and biochemical characterization, the two best bacterial isolates (HCS-1B and WJR-6B) were selected for sclerotinia rot suppression and lettuce growth promotion under greenhouse conditions. The lowest incidence rate (0%) of sclerotinia rot was recorded in WJR-6B-treated lettuce pre-inoculated with S. sclerotiorum and in S. sclerotiorum-inoculated lettuce pretreated with HCS-1B. Combination treatment of HCS-1B and WJR-6B increased the leaf area of lettuce by approximately 3-fold (218.4 cm2) compared to the control (75 cm2). In this study, HCS-1B and WJR-6B were identified as potential antagonistic bacterial isolates that inhibit sclerotinia rot.
Field testing of these potential bacterial isolates would be a good direction for future studies.
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