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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.9
- 수록면
- 127 - 133 (7page)
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We previously showed that γ-glutamyltranspeptidase (GGT), an enzyme involved in glutathione metabolism, in Bacillus subtilis acts as a virulence factor for osteoclastogenesis via the RANKL-dependent pathway. Hence, it can be hypothesized that GGT of periodontopathic bacteria acts as a virulence factor in bone destruction. Because Fusobacterium nucleatum, which is a periodontopathic pathogen, has GGT with a primary structure similar to that of B. subtilis GGT (37.7% identify), the bone-resorbing activity of F. nucleatum GGT was examined here. Recombinant GGT (rGGT) of F. nucleatum was expressed in Escherichia coli and purified using the His tag of rGGT. F. nucleatum rGGT (Fn rGGT) was expressed as a precursor of GGT, and then processed to a heavy subunit and a light subunit, which is characteristic of general GGTs, including the human and B. subtilis enzymes. Osteoclastogenesis was achieved in a co-culture system of mouse calvaria-derived osteoblasts and bone marrow cells. Fn rGGT induced osteoclastogenesis to a level similar to that of B. subtilis rGGT; furthermore, osteoclastogenesis was induced in a dose-dependent manner. These results suggest that F. nucleatum GGT possesses a virulent bone-resorbing activity, which could play an important role in the pathogenesis of periodontitis.
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참고문헌 (21)
참고문헌 신청
Settem RP / 2012 / Fusobacterium nucleatum and Tannerella forsythia induce synergistic alveolar bone loss in a mouse periodontitis model / Infect Immun 80:2436~2443
Polak D / 2009 / Mouse model of experimental periodontitis induced by Porphyromonas gingivalis/Fusobacterium nucleatum infection : bone loss and host response / J Clin Periodontol 36:406~410
Suda T / 1999 / Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families / Endocr Rev 20:345~357
Martin TJ / 1994 / Mechanisms by which cells of the osteoblast lineage control osteoclast formation and activity / J Cell Biochem 56:357~366
Niida S / 2004 / Gamma-glutamyltranspeptidase stimulates receptor activator of nuclear factor-kappaB ligand expression independent of its enzymatic activity and serves as a pathological bone-resorbing factor / J Biol Chem 279:5752~5756
Polak D / 2009 / Mouse model of experimental periodontitis induced by Porphyromonas gingivalis/Fusobacterium nucleatum infection : bone loss and host response / J Clin Periodontol 36:406~410
Suda T / 1999 / Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families / Endocr Rev 20:345~357
Martin TJ / 1994 / Mechanisms by which cells of the osteoblast lineage control osteoclast formation and activity / J Cell Biochem 56:357~366
Niida S / 2004 / Gamma-glutamyltranspeptidase stimulates receptor activator of nuclear factor-kappaB ligand expression independent of its enzymatic activity and serves as a pathological bone-resorbing factor / J Biol Chem 279:5752~5756
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