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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제11호
- 발행연도
- 2018.1
- 수록면
- 1,946 - 1,954 (9page)
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이 논문의 연구 히스토리 (2)
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The aim of this study was to isolate and characterize a lytic Yersinia enterocolitica-specific phage (KFS-YE) as a biocontrol agent. KFS-YE was isolated and purified with the final concentration of (11.72 ± 0.03) log PFU/ml from poultry. As observed by transmission electron microscopy, KFS-YE consisted of an icosahedral head and a contractile tail, and was classified in the Myoviridae family. KFS-YE showed excellent narrow specificity against Y. enterocolitica only. Its lytic activity was stable at wide ranges of pH (4–11) and temperature (4–50°C). The latent period and burst size of KFS-YE were determined to be 45 min and 38 PFU/cell, respectively. KFS-YE showed relatively robust storage stability at -20, 4, and 22°C for 40 weeks. KFS-YE demonstrated a bactericidal effect in vitro against Y. enterocolitica and provided excellent efficiency with a multiplicity of infection as low as 0.01. This study demonstrated the excellent specificity, stability, and efficacy of KFS-YE as a novel biocontrol agent. KFS-YE may be employed as a practical and promising biocontrol agent against Y. enterocolitica in food.
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참고문헌 (41)
참고문헌 신청
Pajunen M / 2000 / Bacteriophage ϕYeO3-12, specific for Yersinia enterocolitica serotype O: 3, is related to coliphages T3 and T7 / J. Bacteriol 182:5114~5120
Salem M / 2015 / Isolation and characterization of Yersinia-specific bacteriophages from pig stools in Finland / J. Appl. Microbiol 118:599~608
Rahman A / 2011 / Yersinia enterocolitica: Epidemiological studies and outbreaks / J. Pathog 211:239391
Harris L / 2003 / Outbreaks associated with fresh produce:incidence, growth, and survival of pathogens in fresh and fresh-cut produce / Compr. Rev. Food Sci. Food Saf 2:78~141
MacDonald E / 2016 / National outbreak of Yersinia enterocolitica infections in military and civilian populations associated with consumption of mixed salad, Norway, 2014 / Euro Surveill 21
Salem M / 2015 / Isolation and characterization of Yersinia-specific bacteriophages from pig stools in Finland / J. Appl. Microbiol 118:599~608
Rahman A / 2011 / Yersinia enterocolitica: Epidemiological studies and outbreaks / J. Pathog 211:239391
Harris L / 2003 / Outbreaks associated with fresh produce:incidence, growth, and survival of pathogens in fresh and fresh-cut produce / Compr. Rev. Food Sci. Food Saf 2:78~141
MacDonald E / 2016 / National outbreak of Yersinia enterocolitica infections in military and civilian populations associated with consumption of mixed salad, Norway, 2014 / Euro Surveill 21
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