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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 806 - 815 (10page)
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초록· 키워드
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This study was performed to isolate some strains of Bifidobacterium breve from fecal materials of neonates and to screen them for the biotransformation activity of converting linoleic acid into conjugated linoleic acid (CLA). Fecal samples were collected from twenty healthy neonates between 14 and 100 days old, and four hundred colonies were randomly selected from a Bifidobacterium selective transoligosaccharide medium. A duplex polymerase chain reaction technique was developed for the rapid and accurate molecular characterization of the B. breve strains that have been reported to show the species-specific characteristic of CLA production. They are identified by 16S ribosomal DNA, fructose-6-phosphate phosphoketolase encoding genes (xfp), and rapid pulsed field gel electrophoresis. Thirty-six isolates were identified as B. breve, and just two of the 12 neonates were harboring B. breve strains. Each isolate showed different CLA-producing ability in the spectrophotometric assay. All of the positive strains from the primary spectrophotometric assay were confirmed for their CLA-producing activities using gas-chromatographic analysis, and their conversion rates were different, depending on the strain isolated in this study. Some strains of B. breve were successfully isolated and characterized based on the CLA-producing activity, and further studies are necessary to characterize the enzyme and the gene responsible for the enzyme activity.
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참고문헌 (38)
참고문헌 신청
Barrett E / 2007 / Rapid screening method for analyzing the conjugated linoleic acid production capabilities of bacterial cultures / Appl Environ Microbiol 73:2333~2337
Berthoud H / 2005 / Comparison of partial gene sequences encoding a phosphoketolase for the identification of bifidobacteria / LWT-Food Sci Technol 38:101~105
Biffi A / 1997 / Antipoliferative effect of fermented milk on the growth of a human cancer cell line / Nutr Cancer 28:93~99
Briczinski EP / 2006 / A rapid pulsed-field gel electrophoresis method for analysis of bifidobacteria / J Dairy Sci 89:2424~2427
Chaplin A / 2015 / Conjugated linoleic acid supplementation under a high-fat diet modulates stomach protein expression and intestinal microbiota in adult mice / PLoS ONE 10:e0125091
Berthoud H / 2005 / Comparison of partial gene sequences encoding a phosphoketolase for the identification of bifidobacteria / LWT-Food Sci Technol 38:101~105
Biffi A / 1997 / Antipoliferative effect of fermented milk on the growth of a human cancer cell line / Nutr Cancer 28:93~99
Briczinski EP / 2006 / A rapid pulsed-field gel electrophoresis method for analysis of bifidobacteria / J Dairy Sci 89:2424~2427
Chaplin A / 2015 / Conjugated linoleic acid supplementation under a high-fat diet modulates stomach protein expression and intestinal microbiota in adult mice / PLoS ONE 10:e0125091
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